Postdoctoral Research Scholar
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Position Number: 00112237
Essential Job Duties:
The Department of Entomology and Plant Pathology at NC State University is seeking a highly motivated Postdoctoral Research Scholar to conduct research focused on the isolation, characterization, genomics, and virulence of Orientia spp. bacteria associated with free-living chiggers.
The successful candidate will work as part of a collaborative research team investigating Orientia spp. through a combination of bacterial culture, cell-based assays, next-generation sequencing, and genomic bioinformatics. The position will involve laboratory research conducted in a BSL-3 facility, as well as computational analysis of bacterial genomes.
Collaborating researchers will collect wild-caught, free-living chiggers in the United States and provide specimens for laboratory analysis. The Postdoctoral Research Scholar will contribute to the culture and characterization of Orientia spp., genomic sequencing and analysis, assessment of bacterial virulence, and preparation of research findings for publication and presentation.
Key Responsibilities:
- Culture Orientia spp. bacteria from specimens collected by collaborating researchers and establish and maintain pure bacterial cultures.
- Conduct laboratory studies to characterize Orientia spp. and assess bacterial virulence using appropriate human or animal cell lines.
- Perform bacterial culture and related laboratory research in a BSL-3 facility in accordance with established biosafety and laboratory protocols.
- Prepare purified bacterial DNA for next-generation sequencing and coordinate genomic sequencing with external sequencing service providers.
- Use probe-directed genome sequencing approaches, as appropriate, to obtain genomic data from Orientia spp. present in crude bacterial mixtures.
- Assemble, annotate, and analyze Orientia spp. genome sequences using bioinformatic approaches.
- Compare newly generated genome sequences with known Orientia spp. genomes to determine species identity and characterize genetic relationships.
- Conduct genomic analyses to investigate genetic factors associated with bacterial virulence and other relevant biological characteristics.
- Integrate laboratory and genomic findings to support research objectives.
- Prepare manuscripts, abstracts, presentations, and other scholarly products for dissemination of research findings.
- Present research findings at scientific meetings and conferences.
- Contribute to collaborative research activities, grant proposals, and other scholarly activities as appropriate.
- Maintain accurate records of laboratory experiments, genomic analyses, and research findings.
1. Culture and characterize Orientia spp. bacteria - 50%
Culture Orientia spp. bacteria from wild-caught, free-living chiggers collected in the United States by collaborating researchers. Establish and maintain pure cultures of Orientia spp. bacteria and conduct laboratory studies to characterize bacterial growth and virulence. Perform bacterial culture and related experimental work in a BSL-3 laboratory using established biosafety protocols. Culture Orientia spp. in appropriate animal cell lines and assess the virulence and biological characteristics of isolated bacterial cultures using human or other animal cell lines. Chiggers will be collected and provided by collaborating researchers in culture media and will not be provided as live specimens.
2. Obtain genomic sequence data for Orientia spp. - 10%
Obtain genome sequence data from Orientia spp. bacteria using next-generation sequencing approaches. Prepare purified DNA obtained from bacterial cultures for submission to an external DNA sequencing service. As an alternative approach, collaborate with private sequencing service providers to use probe-directed genome sequencing to obtain genomic data from Orientia spp. present in crude bacterial mixtures derived from free-living chiggers.
3. Assemble and analyze Orientia spp. genomes - 30%
Assemble and analyze genomic sequence data from Orientia spp. bacteria. Compare newly sequenced genomes with publicly available genomes from known Orientia spp. to determine species identity and characterize genetic relationships. Use genomic and bioinformatic analyses to identify genetic factors associated with bacterial virulence and other relevant biological characteristics. Interpret and integrate genomic findings with laboratory data to support research objectives and prepare results for publication in scientific journals.
4. Research communication and scholarly activities - 10%
Analyze, interpret, and document research findings; prepare manuscripts, abstracts, presentations, and other scholarly materials; contribute to research proposals and grant applications as appropriate; and present research findings at scientific meetings and conferences. Maintain accurate records of experimental procedures, results, and genomic analyses and contribute to collaborative research activities with internal and external research partners.
Departmental Required Skills:
- Ph.D. in Microbiology, Molecular Biology, Genomics, or a related field.
- Experience with mammalian and/or microbial cell culture and standard molecular biology techniques, including DNA extraction, PCR, and next-generation sequencing (NGS).
- Knowledge of microbial genomics, including genome assembly, annotation, and genomic data analysis using bioinformatics tools.
Preferred Experience, Skills, Training/Education:
- Ability to prepare manuscripts and work effectively in a collaborative research environment
Necessary Licenses or Certifications:
Valid NC Driver's License required
AA/EOE:
NC State University is an equal opportunity employer. All qualified applicants will receive equal opportunities for employment without regard to age, color, disability, gender identity, genetic information, national origin, race, religion, sex (including pregnancy), sexual orientation, and veteran status. The University encourages all qualified applicants, including protected veterans and individuals with disabilities, to apply. Individuals with disabilities requiring disability-related accommodations in the application and interview process are welcome to contact 919-513-0574 to speak with a representative of the Office of Equal Opportunity.
If you have general questions about the application process, you may contact Human Resources at (919) 515-2135 or workatncstate@ncsu.edu.
Final candidates are subject to criminal & sex offender background checks. Some vacancies also require credit or motor vehicle checks. Degree(s) must be obtained prior to start date in order to meet qualifications and receive credit.
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Department: 111801 - Entomology and Plant Pathology
Location: Raleigh, NC
