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Position Number: 00107291

Essential Job Duties:
Dr. Piedrahita's laboratory is primarily interested in the behavior of stem and progenitor cells in vitro and in vivo, and the development of large animal models of use in human and veterinary regenerative medicine. Towards this end, his research combines techniques in functional genomics, stem cells, cell biology, embryo manipulation, and molecular biology. The primary purpose of this position is to assist in analyzing a new gene edited pig to study the role of LGR5 cells in multiple organs in particular the lung. Both during development and their involvement in diseases such as IPF, COPD and asthma. The candidate should have a strong background in cell biology, organoid biology, molecular biology and be interested in lung physiology/biology.

Duties and Responsibilities
Assist and lead projects related to the continued improvement of the LGR5 pig model. Lead the analysis of LGR5 stem cells and help elucidate their role in lung development and in repair after injury. Assist in the analysis of single cell RNAseq data to understand the developmental origin of lung LGR5 cells. Utilize organoid and indued pluripotent stem (IPS) cells models to unravel the function of LGR5 cells in the lung. Participate on other laboratory projects as needed.

Additional Duties

• Inventory management main point of contact
• Computing additional needs of the lab including project management of experiments
• Additional duties with compiling publication documents

Departmental Required Skills:
Cell biology, molecular biology, cell culture.

Preferred Experience, Skills, Training/Education:
Organoid biology, stem cell biology including generation of IPS cell lines, lung physiology, gene expression analysis (RNAseq and scRNA seq).

Ability or interest in working with swine as a model system.

Necessary Licenses or Certifications:
N/A

AA/EOE:
NC State University is an equal opportunity employer. All qualified applicants will receive equal opportunities for employment without regard to age, color, disability, gender identity, genetic information, national origin, race, religion, sex (including pregnancy), sexual orientation, and veteran status. The University encourages all qualified applicants, including protected veterans and individuals with disabilities, to apply. Individuals with disabilities requiring disability-related accommodations in the application and interview process are welcome to contact 919-513-0574 to speak with a representative of the Office of Equal Opportunity.

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Department: 194001 - Department of Molecular Biomedical Sciences

Location: Raleigh, NC